Site-selective conjugation of an anticoagulant aptamer to recombinant albumins and maintenance of neonatal Fc receptor binding (1)
Julie Schmøkel, Anders Voldum, Georgia Tsakiridou, Matthias Kuhlmann, Jason Cameron, Esben S Sørensen, Jesper Wengel and Kenneth A Howard
Published 26 April 2017, Nanotechnology 28 (2017)
Publication type: Veltis
"The long circulatory half-life, predominately facilitated by engagement with the cellular recycling neonatal Fc receptor (FcRn), and ligand transport properties of albumin promote it as an attractive candidate to improve the pharmacokinetic profile of aptamers. This study investigates the effect of Cys34 site-selective covalent attachment of a factor IXa anticoagulant aptamer on aptamer functionality and human FcRn (hFcRn) engagement using recombinant human albumin (rHA) of either a wild type (WT) or an engineered human FcRn high binding variant (HB)."