Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents
Barbara Bernardim, Pedro M.S.D. Cal, Maria J. Matos, Bruno L. Oliveira, Nuria Martínez-Sáez, Inês S. Albuquerque, Elizabeth Perkins, Francisco Corzana, Antonio C.B. Burtoloso, Gonzalo Jiménez-Osés & Gonçalo J.L. Bernardes
Nature Communications. 2016 October 26; 7:13128. doi:10.1038/ncomms13128. PMC. WEB 19 Dec. 2016
Pub Type: Data exemplification VELTIS
"Much to our delight, we found that for albumin a single equivalent of 2c is sufficient to achieve complete conversion to the product, which demonstrates the high efficiency of our method"